[Date Prev][Date Next] [Thread Prev][Thread Next] [Date Index] [Thread Index]

Bug#962484: marked as done (ITP: deblur -- deconvolution for Illumina amplicon sequencing)



Your message dated Mon, 12 Feb 2024 04:00:11 +0000
with message-id <E1rZNUJ-00A5MX-3v@fasolo.debian.org>
and subject line Bug#962484: fixed in deblur 1.1.1-1
has caused the Debian Bug report #962484,
regarding ITP: deblur -- deconvolution for Illumina amplicon sequencing
to be marked as done.

This means that you claim that the problem has been dealt with.
If this is not the case it is now your responsibility to reopen the
Bug report if necessary, and/or fix the problem forthwith.

(NB: If you are a system administrator and have no idea what this
message is talking about, this may indicate a serious mail system
misconfiguration somewhere. Please contact owner@bugs.debian.org
immediately.)


-- 
962484: https://bugs.debian.org/cgi-bin/bugreport.cgi?bug=962484
Debian Bug Tracking System
Contact owner@bugs.debian.org with problems
--- Begin Message ---
Package: wnpp
Severity: wishlist

Subject: ITP: deblur -- deconvolution for Illumina amplicon sequencing
Package: wnpp
Owner: Steffen Moeller <moeller@debian.org>
Severity: wishlist

* Package name    : deblur
  Version         : 1.1.0
  Upstream Author : , Deblur development team
* URL             : https://github.com/biocore/deblur
* License         : BSD-3
  Programming Lang: Python
  Description     : deconvolution for Illumina amplicon sequencing
 Deblur is a greedy deconvolution algorithm for amplicon sequencing
 based on Illumina Miseq/Hiseq error profiles.  The authors recommend
 using Deblur via the QIIME2 plugin q2-deblur. Examples of its use can be
 found within the plugin itself. However, Deblur itself does not depend
 on QIIME2.
 .
 The input to Deblur workflow is a directory of FASTA or FASTQ files
 (1 per sample) or a single demultiplexed FASTA or FASTQ file. These
 files can be gzip'd. The output directory will contain three BIOM
 tables in which the observation IDs are the Deblurred sequences. The
 outputs are contingent on the reference databases used and a more
 focused discussion on them is in the subsequent README section titled
 "Positive and Negative Filtering." The output files are as follows:
 .
  * reference-hit.biom : contains only Deblurred reads matching the
    positive filtering database. By default, a reference composed of 16S
    sequences is used, and this resulting table will contain only those
    reads which recruit at a coarse level to it will be retained. Reads
    are also filtered against the negative reference, which by default
    will remove any read which appears to be PhiX or adapter.
 .
  * reference-hit.seqs.fa : a fasta file containing all the sequences
    in reference-hit.biom
 .
  * reference-non-hit.biom : contains only Deblurred reads that did not
    align to the positive filtering database. Negative filtering is also
    appied to this table, so by default, PhiX and adapter are removed.
 .
  * reference-non-hit.seqs.fa : a fasta file containing all the
    sequences in reference-non-hit.biom
 .
  * all.biom : contains all Deblurred reads. This file represents the
    union of the "reference-hit.biom" and "reference-non-hit.biom" tables.
 .
    * all.seqs.fa : a fasta file containing all the sequences in all.biom
 .
 Deblur uses two types of filtering on the sequences:
 .
  * Negative mode - removes known artifact sequences (i.e. sequences
    aligning to PhiX or Adapter with >=95% identity and coverage).
 .
  * Positive mode - keeps only sequences similar to a reference database
    (by default known 16S sequences). SortMeRNA is used, and any sequence
    with an e-value <= 10 is retained. Deblur also outputs a BIOM table
    without this positive filtering step (named all.biom).
 .
 The FASTA files for both of these filtering steps can be supplied via
 the --neg-ref-fp and --pos-ref-fp options. By default, the negative
 database is composed of PhiX and adapter sequence and the positive
 database of known 16S sequences.
 .
 Deblur uses negative mode filtering to remove known artifact (i.e. PhiX
 and Adapter sequences) prior to denoising. The output of Deblur contains
 three files: all.biom, which includes all sOTUs, reference-hit.biom,
 which contains the output of positive filtering of the sOTUs (default
 only sOTUs similar to 16S sequences), and reference-non-hit.biom,
 which contains only sOTUs failing the positive filtering (default only
 non-16S sOTUs).

Remark: This package is maintained by Debian Med Packaging Team at
   https://salsa.debian.org/med-team/deblur

--- End Message ---
--- Begin Message ---
Source: deblur
Source-Version: 1.1.1-1
Done: Andreas Tille <tille@debian.org>

We believe that the bug you reported is fixed in the latest version of
deblur, which is due to be installed in the Debian FTP archive.

A summary of the changes between this version and the previous one is
attached.

Thank you for reporting the bug, which will now be closed.  If you
have further comments please address them to 962484@bugs.debian.org,
and the maintainer will reopen the bug report if appropriate.

Debian distribution maintenance software
pp.
Andreas Tille <tille@debian.org> (supplier of updated deblur package)

(This message was generated automatically at their request; if you
believe that there is a problem with it please contact the archive
administrators by mailing ftpmaster@ftp-master.debian.org)


-----BEGIN PGP SIGNED MESSAGE-----
Hash: SHA256

Format: 1.8
Date: Tue, 30 Jan 2024 20:14:25 +0100
Source: deblur
Binary: deblur
Architecture: source all
Version: 1.1.1-1
Distribution: unstable
Urgency: medium
Maintainer: Debian Med Packaging Team <debian-med-packaging@lists.alioth.debian.org>
Changed-By: Andreas Tille <tille@debian.org>
Description:
 deblur     - deconvolution for Illumina amplicon sequencing
Closes: 962484
Changes:
 deblur (1.1.1-1) unstable; urgency=medium
 .
   * Team upload.
   * Initial release (Closes: #962484)
Checksums-Sha1:
 e191cccef16e0318e6b3eab08ad89d1ba3175d87 2072 deblur_1.1.1-1.dsc
 e6e86817af379b29fbed698573f3463d154a16bf 3901272 deblur_1.1.1.orig.tar.gz
 5e864743071faaea76c470f47770975f1dedf783 3500 deblur_1.1.1-1.debian.tar.xz
 ea73d57efeeb86d7e74671d836005443195ec03f 1730588 deblur_1.1.1-1_all.deb
 95181a891173a7a69739fb0815ef196c1a5c247e 10805 deblur_1.1.1-1_amd64.buildinfo
Checksums-Sha256:
 bdffd98da68882025edce361cefd924137746b3cc963b93d32220c4977087b3f 2072 deblur_1.1.1-1.dsc
 a4d60bde48de1619300a20e689cf80f367c45649f73ce7d196fcfe2db70d3d40 3901272 deblur_1.1.1.orig.tar.gz
 247267fb427ff218c5e848f11534be80312328595d369a4ae1090635c27ed6bd 3500 deblur_1.1.1-1.debian.tar.xz
 fc0f2bfc49ff3ce656f224ff8a433d792c8401c2656a76358d0ba9bd63b9a788 1730588 deblur_1.1.1-1_all.deb
 af9f7f4595cbdcd105800ac70558a04983d85280c97085ed3ce558c7b1e155ee 10805 deblur_1.1.1-1_amd64.buildinfo
Files:
 c0fcacd7d098b36ca3f2d6d5f0d8b3cf 2072 science optional deblur_1.1.1-1.dsc
 38ee00ccde82293c5f122ee36dc4bf25 3901272 science optional deblur_1.1.1.orig.tar.gz
 0bbee26119526a8a910b192360aba89b 3500 science optional deblur_1.1.1-1.debian.tar.xz
 e52a35f10f32a5dca7353347959f467a 1730588 science optional deblur_1.1.1-1_all.deb
 af456e0609ab424ef11b5ec139b7d792 10805 science optional deblur_1.1.1-1_amd64.buildinfo

-----BEGIN PGP SIGNATURE-----
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=lpj6
-----END PGP SIGNATURE-----

Attachment: pgptiFTWUf0U2.pgp
Description: PGP signature


--- End Message ---

Reply to: